■ 基本信息

李毅，重庆医科大学检验医学院特聘教授，博士生导师，致公党员。重庆青年人才专项获得者，新重庆人才C类，重庆市引进高层次人才第四类。曾任澳大利亚生物纳米光子国家卓越中心（CNBP）生物传感课题组负责人、博士生导师，兼任澳洲生物技术公司Avicena System Ltd. 研究顾问。

■ 联系方式

邮箱：yi.li@cqmu.edu.cn

■ 学习经历

2018.03-2022.01，新南威尔士大学 （UNSW）， 澳大利亚，生物医学工程（生物传感），工学博士 （全奖）

2008.01-2010.05，马里兰大学 （UMCP），美国，食品科学（微生物耐药），理学硕士 （全奖）

2002.09-2006.06，中国海洋大学，中国青岛，食品科学与工程，工学学士

■ 工作经历

2023.09-至今，特聘教授，重庆医科大学检验医学院

2023.08-2024.12，技术顾问（兼职），Avicena System Ltd., 澳大利亚

2021.03-2023.09，Research Associate，博士生导师，生物医学工程学院，新南威尔士大学/ 澳大利亚ARC纳米生物光子学卓越研究中心（CNBP）

2013.08-2016.08，官方兽医师，贵州省动物疫病预防控制中心

2011.11-2013.08，技术主管，贵州爱微生物技术有限责任公司

2010.08-2011.07，Research Assistant，美国新墨西哥州VA健康保障系统 / 美国新墨西哥大学医学院

2008.05-2010.06，Research Assistant，美国马里兰大学（UMCP）/ 美国FDA/JIFSAN

■ 研究方向

高性能酶传感技术（CRISPR、pAgo等）

单分子/单细胞快速检测技术

流式微流控平台及信号智能分析

■ 科研项目

1."阅后即焚"式CRISPR单分子核酸检测平台开发, 川渝联合技术创新与应用发展基金（2024TIAD-CYKJCXX0038）, 重庆市科技局, 90万, 项目负责人, 2024.11 – 2027.10

2.Revolutionising single-nucleotide variation detection via digital CRISPR, Australian Research Council, ARC Discovery Project (DP250103029), AU$ 684K, Chief Investigator, 2024.12 – 2027.11

3.Pioneering portable labs: user-friendly molecular level COVID test strips, Australia’s Economic Accelerator (AEA) (AE240300106), AU$ 541K, Chief Investigator, 2024.08 – 2025.07

4.Automated infection diagnostics for safe and healthy communities, CRCAC-Cooperative Research Centres Projects Round 15 (CRCPXV000104), AU$ 7,043K, Chief Investigator, 2024.02 – 2026.01

5.Low-cost biosensors for liquid biopsies: towards practical implementation of precision oncology, NHMRC-Ideas (RG231585/2030464), AU$ 1,144K, Chief Investigator, 2024.02 – 2027.02

6.On-site environmental DNA sensing with user-friendly test strips, Australia, Australian Research Council, ARC Discovery Project (DP240103024), AU$ 603K, Chief Investigator, 2024.02 – 2027.01

7.Novel testing methodologies for deployable detection of water pathogens, Australia, NSW Smart Sensing Network (RG230538), AU$ 200K, Chief Investigator, 2023.12 – 2024.12

■ 学术论著

已在Nature Communications, Water Research, Sensors and Actuators: B. Chemical, Trends in Biotechnology等国际学术期刊发表SCI论文20余篇，谷歌引用 >1600次，ESI高被引2篇。

1.Xuan Wu, Ningning Pi, Fei Deng, Shiyang Tang, Chengchen Zhang, Lu Zhu, Fangfang Sun, Xiaoyao He, Hanqing Li, Shilong Zhao, Rong Xiang,Yi Li*, Circular DNA enhanced amplification-free CRISPR/Cas12a assays for end-user friendly ultra-sensitive Porphyromonas gingivalis diagnosis.Microchemical Journal, 207, 111850, 2024. (IF=4.9)

2.Xuan Wu, Rong Xiang, Danting Yang, Xiaoyao He, Lu Zhu, Fangfang Sun, Hanqing Li, Ningning Pi,Yi Li*, HRP-Integrated CRISPR/Cas12a Biosensor for Rapid Point-of-Care Detection of Langya Henipavirus.iScience, 27, 12, 111466, 2024. (IF=4.6)

3.Fei Deng,Yi Li*(共一、唯一通讯), Biyao Yang, Rui Sang, Wei Deng, Maya Kansara, Frank Lin, Subo Thavaneswaran, David M. Thomas, Ewa M. Goldys, Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing.Nature Communications, 2024. (IF=14.7, ESI高被引)

4.Fei Deng,Yi Li*(共一、通讯), Tim Hall, Graham Vesey, Ewa M. Goldys*, Bi-functional antibody-CRISPR/Cas12a ribonucleoprotein conjugate for improved immunoassay performance.Analytica Chimica Acta, 1259, 341211, 2023. (IF=6.911)

5.Yi Li*, Linyang Liu, Laicong Qiao, Fei Deng, Universal CRISPR/Cas12a-associated aptasensor suitable for rapid detection of small proteins with plate reader.Frontiers in Bioengineering and Biotechnology, 1201175, 2023. (IF=6.064)

6.Yi Li*, Fei Deng, Chengchen Zhang, Xiaofen Lin, Ewa Goldys, Integrated RPA-CRISPR/Cas12a system towards Point-of-Care H. pylori detection.IEEE Engineering in Medicine & Biology, 2023.

7.Fei Deng,Yi Li*(共一、通讯), Bitong Li, Ewa M. Goldys*, Increasing trans-cleavage catalytic efficiency of Cas12a and Cas13a with chemical enhancers enables amplified nucleic acid detection.Sensors and Actuators: B. Chemical, 373, 132767, 2022. (IF=9.221)

8.Fei Deng, Laicong Qiao,Yi Li*, A fluorescent immunosensor on optical fibre for the multiplex detection of proinflammatory cytokines.Sensing and Bio-Sensing Research, 37, 100501, 2022. (IF=4.461)

9.Yi Li*, Fei Deng, Ewa M. Goldys*, A simple and versatile CRISPR/Cas12a-based immunosensing platform: Towards attomolar level sensitivity for small protein diagnostics.Talanta, 4, 13, 123469, 2022. (IF=6.556)

10.Fei Deng,Yi Li*(共一、通讯), Laicong Qiao, Ewa Goldys*, A CRISPR/Cas12a-assisted on-fibre immunosensor for ultrasensitive small protein detection in complex biological samples.Analytica Chimica Acta, 4, 12, 339351, 2021. (IF=6.911)

11.Yi Li, Fei Deng, Tim Hall, Graham Vessey, Ewa M. Goldys*, CRISPR/Cas12a-powered immunosensor suitable for ultra-sensitive whole Cryptosporidium oocyst detection from water samples using a plate reader.Water Research, 203, 117553, 2021. (IF=13.4)

12.Yi Li, Fei Deng, Ewa M. Goldys*, A versatile CRISPR/Cas12a-based sensitivity amplifier suitable for commercial HRP-based ELISA kits.Sensors and Actuators: B. Chemical, 347, 130533, 2021. (IF=9.221)

13.Yi Li, Linyang Liu, Guozhen Liu*, CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?Trends in Biotechnology, 37 (8): 792-795. 2019. (IF=21.942)

14.Yi Li, Shiyuan Li, Jin Wang, Guozhen Liu*, CRISPR/Cas Systems towards Next-Generation Biosensing.Trends in Biotechnology, 37 (7): 730-743. 2019. (IF=21.942, ESI-高被引)

■ 专利授权

中国专利申请5项，澳洲PCT专利2项，授权企业转化2项。