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专职教师

杨晓兰 教授,博士生导师

2020年01月13日 13:58  点击:[]

杨晓兰,女,1968年出生,药理学博士,教授,博士生导师

联系信息重庆医科大学检验医学院临床检验诊断学教育部重点实验室

Tel, +86-23-68485240; Fax, +86-23-68485240; E-mail, xiaolanyang666@yeah.net

http://orcid.org/0000-0001-9320-1036

http://www.researchgate.net/profile/Xiaolan_Yang3

http://jyx.cqmu.edu.cn/s/47/t/141/14/9c/info5276.htm


学习(进修)与工作经历

1987.9~1991.7,获西南师范大学化学系理学学士学位

1991.9~1994.7,获北京师范大学有机化学专业理学硕士学位

2003.9~2006.7,获重庆医科大学药理学专业博士学位

1994.7~2003.7,重庆医科大学基础医学院化学教研室任教

2003.7~2010.12,重庆医科大学药学院药物化学教研室任教

2011.1~至今,重庆医科大学检验医学院、临床检验诊断学教育部重点实验室教授、硕士生导师、博士生导师


研究方向:蛋白配体设计合成、高通量筛选与分离分析材料:

1. 同工酶的选择性抑制剂的设计合成与筛选,生物活性评价;

2. 蛋白质标记、修饰与位点选择性固定化;

3. 磁珠固定化靶蛋白或配体制备分离分析复合材料;

4. 磁分离选择性指数富集快速筛选组合合成配体混合物、中药方剂及天然产物混合物中高亲和力配体。


作为负责人主持的科研项目

2016/01-2019/12,国家自然科学基金面上项目“混合物中低丰度高亲和力配体的指数富集技术及应用”,编号31570862。

2012/09-2015/09,重庆市科委面上项目“融合表达标签GST亲和标记试剂设计筛选与应用”,编号CSTC2012JJA0057。

2011/09-2012/12,国家高技术研究发展计划(863计划,编号2011AA02A108)子课题6“新型试剂材料制备”。

2010/06-2012/12,重庆市教委项目“聚乙二醇包被磁性微球制备及其在配体混合物组合库筛选中的应用”,编号KJ100313。


发表的主要论文# 标注为共同第一作者,* 标注为通讯作者)

1. XiangZhang, Shu He, Xiaolei Hu, Jing Wu, Xinpeng Li, Fei Liao*, Xiaolan Yang*(co-corresponding author). Comparison of the Full-Length and 152~528 Truncate of Human Cyclic Nucleotide Phosphodiesterase 4B2 for the Characterization of Inhibitors.Comb Chem High Throughput Screen.2019;22 (1):49-58.

2. YapingLi, Huimin Chong, Xiang Zhang,Xiaolan Yang*, Fei Liao*. High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag,Anal Biochem,2020; 588:113474

3. Jingjing Rao, Juan Liaob, Youquan Bu, Yitao Wang, Xiaolei Hu, Gaobo Long, MingtongHuang, Luhui Zhong, Xiaolan Yang*, Fei Liao*. Ampholytic ion-exchange materials coated with small zwitterions for high-efficacy purification of ionizable soluble biomacromolecules.,Int.J. Biol. Macromol.,2018,120:2234–2241,

4. Mingyue Zou, Xiaolei Hu, Bangtian Xu, Tingting Tong, Yixian Jing, Lei Xi,Wei Zhou, Jingpeng Lu, Xin Wang, Xiaolan Yang*, Fei Liao*. Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer,Oncology Reports2018,41(2):989-998

5. Cong Luo,Xiaolan Yang, Ming Li, Hua Huang, Quan Kang, Xiaobo Zhang, Hui Hui, Xin Zhang,Chaode Cen, Yujia Luo, Lina Xie, Changxuan Wang, Tongchuan He, Dianming Jiang, Tingyu Li and Hong An. A novel strategy for in vivo angiogenesis and osteogenesis: magnetic micro-movement in a bone scaffold,Artif Cell Nanomed B, 2018, 46(sup2):636-645

6. YangXiaolan, Feng Yiran, Chong Huimin, Wang Deqiang, Hu Xiaolei, Pu Ju, Zhan Chang-guo, Liao Fei*. High-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins. Anal Biochem. 2017 Oct 1;534:91-98

7. Yiran Feng#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Huimin Chong, Juan Liao, Chang-guo Zhan, Fei Liao*. Polyclonal Antibodies in Microplates to Predict the Maximum Adsorption Activities of Enzyme/Mutants from Cell Lysates.Protein J, 2017,36:212-219

8. Tao Feng#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Juan Liao, Jun Pu, Xinyun Zhao, Chang-Guo Zhan, Fei Liao*. A Practical System for High-Throughput Screening of Mutants of Bacillus fastidiosus Uricase.Appl Biochem Biotechnol. 2017, 181(2):667-681

9. Jun Pu, Xiaolei Hu, Juan Liao, Yuwei Li, Jialin Qin, Yanling Xie, Chang-Guo Zhan, Xiaolan Yang*, Fei Liao*(co-corresponding author). Achievement of linear response for competitive bioaffinity assay of ligand: criteria of optimized interaction system.RSC Adv 2016, 6(112): 110858-110865

10. Jing Wu#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Juan Liao, JingJing Rao, Jun Pu, Chang-guo Zhan, Fei Liao*. A numerical approach for kinetic analysis of the nonexponential thermoinactivation process of uricase.Protein J 2016, 35(4): 318-329 (DOI: 10.1007/s10930-016-9675-9)

11. Chun Zhang#, Jun Pu#, Xiaolan Yang#, Tao Feng, Fang Liu, Deqiang Wang, Xiaolei Hu, Ang Gao, Hongbo Liu, Chang-Guo Zhan*, Fei Liao*(co-correspondingauthor). Extracorporeal delivery of a therapeutic enzyme.Sci Report 2016; 6:30888 Yuwei Li#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Mei Yuan, Jun Pu, Chang-Guo Zhan,ZhaoyongYang, Fei Liao*. Striking Effects of Storage Buffers on Apparent Half-Lives of the Activity of Pseudomonas aeruginosa Arylsulfatase. Protein J. 2016 Jul 2. (DOI 10.1007/s10930-016-9671-0)

12. Mei Yuan#, Xiaolan Yang#, Yuwei Li, Hongbo Liu, Jun Pu, Chang-Guo Zhan, Fei Liao*. Facile alkaline lysis of Escherichia coli cells in high-throughput mode for screening enzyme mutants: arylsulfatase as an example. Appl Biochem Biotechnol 2016; 179:545-557 (DOI: 10.1007/s12010-016-2012-0).

13. Liqing Tan#, Tao Jiang#,XiaolanYang#, Wei Li, Lijun Pan,Mingan Yu*.Core–shell biopolymer microspheres for sustained drug release. J Appl Polym Sci 2015,132, 41782 (DOI: 10.1002/app.41782)

14. Xiaolan Yang, Xiaolei Hu, Chunyan Chen, Gaobo Long, Jing Wu, Deng Tan, Yuanli Li, Yiran Feng, Jun Pu, Fei Liao*. Comparison of the immobilization of 6His-tagged proteinsonmagnetic-submicron-particle functionalized with Ni2+-NTA and bis-sulfone.Nanosci Nanotechn Lett,2015, 7(6):486-494.

15. Juan Feng#, Lu Wang#, Hongbo Liu#, Xiaolan Yang, Lin Liu, Yanling Xie, Miaomiao Liu, Yunsheng Zhao, Xiang Li, Deqiang Wang, Chang-Guo Zhan, Fei Liao*. Crystalstructureof Bacillus fastidious uricase reveals an unexpected folding of the C-terminus residues crucial for thermostability under physiological conditions.Appl Micriobiol Biotechnol2015;99:7973–7986.

16. Hongbo Liu#, Mei Yuan#, Xiaolan Yang#, Xiaolei Hu, Jizheng Dang, Yanling Xie, Jun Pu, Yuanli Li, Chang-Guo Zhan, Fei Liao*. Comparison of candidate pairs of hydrolytic enzymes for spectrophotometric-dual- enzyme-simultaneous-assay.Anal Sci2015, 31(5):421-427.

17. Yuanli Li#, Gaobo Long#, Xiaolan Yang#, Xiaolei Hu, Yiran Feng, Deng Tan, Yanling Xie, Jun Pu, Fei Liao*.Approximated maximum adsorption of His-tagged enzyme/mutants on Ni2+-NTA for comparison of specific activities.Int J Biol Macromol2015; 74: 211-217.

18. Ya Huang#, Yuanxiang Chen#, Xiaolan Yang#, Hua Zhao, Xiaolei Hu, Jun Pu, Juan Liao, Gaobo Long, Fei Liao*. Optimization of pH values to formulate the bi-reagent kit for serum uric acid assay.Biotechnol Appl Biochem2015, 62(1):137-144. 6. BangtianXu#, Deng Tan#, Xiaolan Yang#, Xiaolei Hu, Yanling Xie, Jialin Qin, Chunyan Chen, Chenxiong He, Yuanli Li, Jun Pu, Fei Liao*. Fluorometric titration assay of affinity of tight-binding nonfluorescent inhibitor of glutathione s-transferase.J Fluoresc2015, 25(1):1-8

19. Core–shell biopolymer microspheres for sustained drug release.J Appl Polym Sci.2015April,132(14):41782

20. Xiaolan Yang, Xiaolei Hu, Bangtian Xu, Xin Wang, Jialin Qin, Chenxiong He, Yanling Xie, YuanliLi, Lin Liu, Fei Liao*. Fluorometric titration approach for calibration of quantity of binding site of purified monoclonal antibody recognizing epitope/hapten nonfluorescent at 340 nm.Anal Chem.2014, 86(12):5667-5672

21. Yuanli Li#, Xiaolan Yang#, Chenxiong He, Xiaolei Hu, Jun Pu, Lin Liu, Gaobo Long, Fei Liao*.Facile quantitative comparison of specific activities of fusion-tagged enzyme/mutants in cell lysates via prediction of their maximum adsorption by anti-tag antibody immobilized in microplate wells.RSC Advance.2014, 4(56): 29925-29932

22. Chun Zhang#, Xiaolan Yang#, Ang Gao, Xiaolei Hu, Jun Pu, Hongbo Liu, Juan Feng, Juan Liao, Yuanli Li, Fei Liao*.Comparison of modification of a bacterial uricase with N-hydroxysuccinimide esters of succinate and carbonate ofmonomethoxylpoly(ethylene glycol).Biotechnol Appl Biochem. 2014, 61(6):683-690

23. Jialin Qin#, Yuanli Li#, Chenxiong He#, Xiaolan Yang, Yanling Xie, Xiaolei Hu, Chunyan Chen, Lu Wang, Jun Pu, Fei Liao*.Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor. Anal Chim Acta. 2014, 829(1):60-67.

24. Chunyan Chen#,MiaomiaoLiu#,Jing Wu#,Xiaolan Yang,Xiaolei Hu,Jun Pu,Gaobo Long,Yanling Xie,Hairong Jiang,Yonghua Yuan, Fei Liao*.Microplate-based method to screen inhibitors of isozymes of cyclic nucleotide phosphodiesterase fused to SUMO. J Enzym Inhib Med Ch. 2014.;29(6):836-839.

25. Xiaolan Yang, Zhiyin Du, Jun Pu, Hua Zhao, Hong Chen, Yin Liu, Zhirong Li, Zhenli Cheng, Huansi Zhong, Eei Liao*.Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition type.J Enzyme Inhibit Med.Chem.2013,28(1):205-213.

26. Hongbo Liu#, Xiaolan Yang#, Lin Liu, Jizheng Dang, Yanling Xie, Yi Zhang, Jun Pu, Gaobo Long, Yuanli Li, Yonghua Yuan, Juan Liao, Fei Liao*Spectrophotometric-dual-enzyme-simultaneous-assay in one reaction solution (SDESA): chemometrics and experimental models.Anal Chem. 2013, 85 (4):2143-2154.

27. Yi zhang#, Xiaolan Yang#, Lin Liu, Zuexia Huang, Jun Pu, Gaobo Long, Ling Zhang, Dong Liu, Bangtian Xu, Juan Liao, Fei Liao*.Comparison of FÖrster-resonance-energy-transfer acceptors for tryptophan and tyrosine residues in native proteins as donors.J Fluorescence2013, 23(1):147-157.

28. Hongbo Liu#, Xiaolan Yang#, Jizheng Dang, Lin Liu, Xiaolei Hu, Jun Pu, Gaobo Long and Fei Liao*.Two glycosidases as label enzymes for concurrent enzyme-linked-immunosorbent-assay of two components via spectrophotometric-dual-enzymesimultaneous-assay in one solution.Anal Methods.2013, 5(10): 5969-5976.

29. Gaobo Long#, Xiao-lan Yang#, Yi Zhang, Jun Pu, Lin Liu, Hong-bo Liu, Yuan-li Li, Fei Liao*.Facile one-step coating approach to magnetic submicron particles withpoly(ethylene glycol) coats and abundant accessible carboxyl groups.Int J NanoMed.2013, 8:791-807.

30. Gaobo Long#, Yi Zhang#, Xiaolan Yang#, Jun Pu, Jialin Qin, Lin Liu, Fei Liao*.Facilecharacterizationof the immobilization of streptavidin on magnetic submicron particles with a fluorescent probe of streptavidin.Appl Spectros.2013, 67(6):688-691.

31. Yi Zhang#, Xiaolan Yang#, Xiaolei Hu#, Miaomiao Liu, Chunyan Chen, Yanling Xie, Jun Pu, Jing Wu, Gaobo Long, Fei Liao*.Resonant-Mie- scattering of aggregates of phosphomolybdate and papaverine for measuring activities and screening inhibitors of cyclic nucleotide phosphodiesterase isozymes.Anal Chim Acta2013, 804:215-220.

32. Jizheng Dang#, Hongbo Liu#, Xiaolan Yang#, Yi Zhang, Yanling Xie, Yuanli Li, Jun Pu, Juan Liao,YonghuaYuan, Fei Liao*. Chromogenic substrate from 4-nitro-1-naphthol for hydrolytic enzyme of neutral or slightly acidic optimum pH: 4-nitro-1-naphthyl-β-D-galactopyranoside as an example.Bioorg Med Chem Lett.2013, 23(3):646-649.

33. Xiaolan Yang, Yonghua Yuan, Chang-Guo Zhan*, Fei Liao* Uricases as therapeutic agents to treat refractory gout: current states and future directions.Drug Dev Res.2012, 73(2):66-72

34. Xiaolan Yang, Jun Pu, Hua Zhao, Xiaoyan Li, Juan Liao, Yanling Xie, Sha Zhu, Gaobo Long, YonghuaYuan, Fei Liao.Method to screen aromatic ligands in mixtures for quantitative affinities to target using magnetic separation of bound ligands along with HPLC and UV photometry detection.Michrochim Acta.2012, 176(1):243-249.

35. Ang Gao#, Xiaolan Yang#, Chun Zhang, Gaobo Long, Jun Pu, Yonghua Yuan, Gaobo Long, Yuanli Li, Fei Liao*.Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives.Chem Cent J.2012, 6(1):142

36. Chun Zhang, Xiao-lan Yang, Yong-hua Yuan, Jun Pu, Fei Liao*.Site-specific PEGylation of therapeutic proteins via optimization of both accessible reactive amino acidresiduesand PEG derivatives.Biodrugs.2012 Aug 1;26(4):209-215.

37. Xiaolan Yang,Gaobo Long,Hairong Jiang,Pu Liao,Fei Liao*.Integration of kinetic analysis of reaction curve with a proper classical approach for enzymatic analysis.Scientific WorldJournal.2012 May3(Epub) ,2012:969767.

38. Xiaolan Yang, Yanling Xie , Jun Pu, Hua Zhao, Juan Liao, Yiwen Chen, Gaobo Long, Chun Zhang, Yonghua Yuan, Sha Zhu, Huidong Yuan, Fei Liao*. Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model.BMC Biotechnol.2011, 11:44

39. Xiaolan Yang#,BeizhongLiu#, Yu Sang, Yonghua Yuan, Jun Pu, Yin Liu, Zhirong Li, Juan Feng, Yanling Xie, Renkuan Tang, Huidong Yuan, Fei Liao*. Kinetic analysis of lactate-dehydrogenase-coupled reaction process and measurement of alanine transaminase by an integration strategy.Anal Sci. 2010, 26(11):1193-1198.

40. Yanling Xie#, Xiaolan Yang#, Jun Pu, Yunsheng Zhao, Ying Zhang, Guoming Xie, Jun Zheng, Huidong Yuan, Fei Liao*.Homogeneous competitive assay of ligand affinities based on quenching fluorescence of tyrosine/tryptophanresiduesin a protein via FOrster-resonance-energy -transfer.Spectrochim Acta A Mol Biomol Spectrosc.2010, 77(4):869-876.

41. Chun Zhang#, Xiaolan Yang#, Juan Feng, Yonghua Yuan, Xiang Li, Youquan Bu, Yanling Xie, Huidong Yuan, Fei Liao*. Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus.Biosci Biotechnol Biochem 2010, 74(6): 1298-1301.

获得授权的中国发明专利

1. 杨晓兰;夏超;廖飞;胡小蕾;张祥,标签GST的一种不可逆亲和标记试剂及应用,中国发明专利,ZL CN201710000094.6,授权通知2020-01-02

2. 杨晓兰,何姝,廖飞,胡小蕾,张祥,陈春艳。异香兰酸衍生物与饱和小环胺缩合所得苯甲酰胺类人PDE4B抑制剂。中国发明专利,ZL 201610093456.6,授权公告号CN105646399B,授权日期2019-03-08

3. 一种均相定量比较未纯化酶及其突变体的方法,2018.08.10,国家发明专利,第二发明人。

4. 在竞争性抑制剂保护下用针对氨基的活化聚乙二醇修饰尿酸酶的方法,2018.07.06,国家发明专利,第二发明人。

5. 双-对烷氧基苯丙烯酮类谷胱甘肽-S-转移酶潜抑制剂,2018.03.27,国家发明专利,第二发明人。

6. Liao Fei, Yang Xiaolan, Liu Hongbo, Dang jizheng, Long Gaobo, Li Yuanli, Liu Lin. Method for simultaneouslymeasuringactivity of various enzymes by using multiple-wavelength absorption single channel. US9611502B2, Date of patent, April 4, 2017.

7. 廖飞,杨晓兰,胡小蕾,秦家林,何琛雄,李元丽,刘霖。一种荧光滴定测定单抗活性位点浓度的方法。中国发明专利,ZL 201410190645.6.授权公告号:CN103954599B,日期2016-6-8

8. 廖飞,杨晓兰,胡小蕾,李元丽,陈春艳,刘霖。一种快速比较未纯化融合酶及其突变体比活性的方法。中国发明专利,ZL 201410190677.6.授权公告号:CN103954762B,日期2016-5-30

9. 廖飞,杨晓兰,胡小蕾,黄亚,陈远翔,李元丽。一种双试剂法测定尿酸含量试剂盒的配方。中国发明专利,ZL201310591044.1;授权公告号CN103571916B,日期2016-03-16.

10. 廖飞,杨晓兰,党纪政,刘红博,张奕,李元丽。一种用4-硝基-1-萘酚或其衍生物为显色团制备b-半乳糖苷酶显色底物的方法及其应用。中国发明专利,ZL 201210461131.0。授权公告号:CN102977160B,日期2016-01-06.

11. 廖飞,杨晓兰,刘红博,党济政,龙高波,李元丽,刘霖。用多波长吸收单通道同步测量多种酶活性的方法。中国发明专利,ZL201210355606.8;授权公告号:CN102879343B,日期2014-10-29

12. 廖飞,杨晓兰,龙高波,蒲军,刘红博,李元丽。用两端带羧基的直链亲水聚合物包被纳米磁芯制备微纳米磁性材料的方法及其应用。中国发明专利,ZL 201210046309.5.授权公告号:CN102617810B,日期2014-09-24. IPC分类号C08F290/06(2006.01)I

13. 廖飞,杨晓兰,龙高波,刘红博,廖娟。联用酶反应动力学过程分析法和终点平衡法测定酶底物量的方法。中国发明专利,ZL 201110321126.5.授权公告号:CN102507470B,日期2014-09-24. IPC分类号G01N21/31(2006.01)I

14. 廖飞,杨晓兰,袁拥华,赵运胜,冯娟,张纯,谢燕玲。作用于血液中低分子物质的大分子药物的体外给药装置及其应用。中国发明专利,ZL 200910251060.X;授权公告号:CN102107032B,日期2014-08-27. IPC分类号A61M1/36(2006.01)I

15. 廖飞,赵运胜,杨晓兰,桑宇,谢艳玲,廖娟,刘北忠,于明安。用偶联酶的最大瞬时速度测定靶酶活性的方法。中国发明专利ZL 200910104280.X,授权公告号:CN101597636B,日期2012-10-31. IPC分类号C12Q1/00(2006.01)I

16. 廖飞,杨晓兰,谢燕玲,邓萍,赵运胜,朱莎,甘志勇,廖娟,刘冬,于明安。联用磁分离与色谱分析测定混合物中配体亲和力的方法。中国发明专利,ZL 200810237298.2,授权公告号:CN101620205B,日期2012-09-05. IPC分类号G01N30/00(2006.01)I

17. 廖飞,赵运胜,杨晓兰,李想,张纯,冯娟,卜友泉,谢燕玲。一种重组表达苛求芽孢杆菌胞内尿酸酶及其聚乙二醇修饰与应用。中国发明专利,ZL200910103741.1,授权公告号:CN101875922B,日期2012-02-22. IPC分类号C12N9/06(2006.01)I

专著、教材

1. Xiaolan Yang, Gaobo Long, Hua Zhao and Fei Liao*. Kinetic Analyses of Enzyme Reaction Curves with New Integrated Rate Equations and Applications, inChemometrics in Practical Applications(2012). pp.157-184. Kurt Varmuza (Ed.), ISBN: 978-953-51-0438-4, InTech

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